全文获取类型
收费全文 | 67708篇 |
免费 | 19196篇 |
国内免费 | 27842篇 |
专业分类
林业 | 7575篇 |
农学 | 6849篇 |
基础科学 | 8152篇 |
34031篇 | |
综合类 | 29858篇 |
农作物 | 6277篇 |
水产渔业 | 5850篇 |
畜牧兽医 | 7621篇 |
园艺 | 2392篇 |
植物保护 | 6141篇 |
出版年
2024年 | 483篇 |
2023年 | 1000篇 |
2022年 | 1227篇 |
2021年 | 1075篇 |
2020年 | 2721篇 |
2019年 | 6177篇 |
2018年 | 5977篇 |
2017年 | 6747篇 |
2016年 | 7627篇 |
2015年 | 7605篇 |
2014年 | 6697篇 |
2013年 | 6983篇 |
2012年 | 6754篇 |
2011年 | 5669篇 |
2010年 | 4636篇 |
2009年 | 4138篇 |
2008年 | 3071篇 |
2007年 | 3216篇 |
2006年 | 2881篇 |
2005年 | 2604篇 |
2004年 | 2647篇 |
2003年 | 2581篇 |
2002年 | 2596篇 |
2001年 | 2097篇 |
2000年 | 1614篇 |
1999年 | 1456篇 |
1998年 | 1250篇 |
1997年 | 1271篇 |
1996年 | 1115篇 |
1995年 | 1164篇 |
1994年 | 1157篇 |
1993年 | 912篇 |
1992年 | 925篇 |
1991年 | 777篇 |
1990年 | 604篇 |
1989年 | 594篇 |
1988年 | 538篇 |
1987年 | 389篇 |
1986年 | 397篇 |
1985年 | 212篇 |
1984年 | 196篇 |
1983年 | 190篇 |
1982年 | 190篇 |
1981年 | 230篇 |
1980年 | 192篇 |
1979年 | 151篇 |
1978年 | 130篇 |
1975年 | 109篇 |
1965年 | 137篇 |
1964年 | 140篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
SMD残留检测的ELISA方法的建立和初步应用 总被引:12,自引:0,他引:12
用戊二醛法将SMD与载体蛋白BSA偶联 ,制备合成抗原SMD BSA作免疫原 ,同法合成包被抗原SMD OVA ,免疫健康家兔获得抗血清。分别用双向琼脂扩散试验和ELISA试验对抗血清进行定性定量测定 ,结果表明所获抗血清特异性针对SMD。用所制备的抗血清建立间接竞争ELISA方法。优化了ELISA的工作条件 ,方阵测定确定了包被抗原最佳浓度 (50 μg/mL) ,抗血清最佳稀释度 (1∶1 0 0 ) ,酶标抗体的最适工作稀释度 (1∶50 0 ) ,并建立了ELISA标准工作曲线。工作曲线表明在 1 0~ 2 0 0 0 μg/L浓度范围内呈良好的线性关系。该法检测底限为 63μg/L,低于国际规定残留限量 (1 0 0 μg/kg)和国内规定残留限量 (30 0 μg/kg)的要求。本法同时测定了回收率以及实际样品血清中的SMD的残留含量 相似文献
102.
目的:比较多层螺旋CT(multi-slice spiral CT,MSCT)与核磁共振血管成像(MRA)、数字减影(DSA)对主动脉夹层(aortic dissection,AD)的诊断价值。方法:对17例AD患者行MSCT检查,并与MRA、DSA的检查结果进行比较。结果:对17例AD患者用MSCT、MRA、DSA都能作出诊断(按DeBakey分型,Ⅰ型有4例,Ⅱ型有1例,Ⅲ型有12例),但只有MSCT能明确显示夹层的范围和分支的受累情况,以及剥离内膜瓣、裂口及主动脉的周围情况。而MRA对裂口的检出率只有21.74%,DSA可达87.0%。结论:MSCT对AD的分型以及破裂口的定位诊断更具准确性,对动脉分支显示更清晰。 相似文献
103.
M E Olson C J Hannigan P F Gaviller L A Fulton 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):865-868
Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs. 相似文献
104.
Χ灾绷吹矸酆康挠跋焓钦?,直接影响比间接影响大。Study on the correlation between rice quality and agronomic traits of quality riceNIE Cheng rong, WEN Yu hui, WANG Yun bo, CHEN Ming zhong,LI Mei, LIU Dao quan(Agronomy Department, Foshan University , Nanhai 528231,China)The correlative analy 相似文献
105.
DONOSO Leonito Acosta 《中国林业科技(英文版)》2005,(1)
The Luntiang Kamaynilaan Project (Metro ManilaGreening Project) launched in 1988 planted trees inmajor thoroughfares, center islands, school premisesand other idle public lands, was basically a governmenteffort. When the funds ran out, the project also fizzledout. In both projects however, it is evident that the keyfactor is peoples participation.Gaining lessons from the past two projects, thegovernment established a collaborative effort ofgovernment and non-government organizations. TheCl… 相似文献
106.
3种农药的微核效应研究 总被引:6,自引:0,他引:6
运用蚕豆根尖微核技术研究3种农药(甲胺磷、甲基硫菌灵和盐酸吗啉胍)在不同浓度下的诱变效应。结果表明,3种农药诱发蚕豆根尖细胞微核率与对照组相比均有不同程度的上升(P<0.05或P<0.01),农药浓度与微核率呈剂量-效应关系,盐酸吗啉胍的诱变效应大于其他2种农药。 相似文献
107.
108.
E M Aburto A Cribb I C Fuentealba B O Ikede F S Kibenge F Markham 《Canadian journal of veterinary research》2001,65(2):104-110
This study evaluates the ability of selenium (Se) supplementation to prevent experimental copper (Cu)-induced hepatocellular damage. Weanling male Fischer 344 rats were randomly assigned to groups of 15, 3 groups (A,B,C) were fed Cu-loaded diets (containing 2000 microg/g copper, added as CuSO4) and different levels of Se (added as Na2SeO3 x 5H2O) as follows: A) Cu-loaded/Se adequate diet (0.4 microg/g Se, fed basis); B) Cu-loaded/Se-supplemented diet (2 microg/g Se); and C) Cu-loaded/Se-deficient diet (< 0.2 microg/g). Three additional groups (D,E,F) were fed diets containing adequate levels of Cu (14 microg/g Cu, fed basis) and different levels of Se as follows: D) Cu-adequate/Se-adequate diet; E) Cu-adequate/Se-supplemented diet (2 microg/g Se); and F) Cu-adequate/Se-deficient (< 0.2 microg/g) diet. After 4, 8, and 12 weeks on the experimental diets, liver samples were processed for histology, histochemistry, metal analysis, glutathione peroxidase (GSH-Px) measurement, and quantification of malondialdehyde (MDA). Morphologic changes characteristic of Cu-associated hepatitis, without an increase in hepatic MDA levels, were seen in all Cu-loaded rats in each sampling. Similar changes occurred in rats fed Se-adequate, Se-supplemented and Se-deficient diets. This study demonstrates that Fischer 344 rats fed 2000 microg/g Cu develop morphologic changes due to Cu toxicity without evidence of lipid peroxidation. Furthermore, Se supplementation does not result in protection against Cu-induced liver injury. 相似文献
109.
杨志清 《云南农业大学学报(自然科学版)》2002,17(2):114-116
以狗牙根和草地早熟禾为材料,用二因素二次正交旋转设计,对9个不同播种比例混播草坪的种群性状进行研究,建立了草坪地上生物量、地下生物量及一致性与狗牙根和草地早熟禾播种量之间显著相关的回归模型。确定了影响草坪性状的主要因子,优化了混播草坪的最佳播种量。 相似文献
110.
Differences in Mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. Forty-six pigs were used, including a treatment group and positive and negative control groups. The pigs were intratracheally inoculated with an M. hyopneumoniae suspension or with Friis media as a placebo. To evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested by nested polymerase chain reaction (N-PCR). Temperature, weight and circulating antibodies were measured for 35 days. At 11 and 35 d postinoculation the pigs were necropsied and macroscopic and microscopic lesions were determined. A section of bronchus was tested by the indirect immunofluorescence test (IFAT), scanning electron microscopy (SEM) and N-PCR. The N-PCR results from the nasal and tracheal swabs showed that the pigs sired by one boar (B3) had a distinctive colonization pattern, different from that of the pigs from the other 2 boars and from the positive controls. SEM studies demonstrated that at 35 d postinoculation a higher proportion of B3 pigs had lower numbers of mycoplasmas attached to the cilia compared with B1 and B2 offspring. No significant differences were observed in temperature and weight gain among groups by ANOVA; however, with use of a 2 × 2 table, temperature differences were observed between pigs sired by boars B1 and B2 at 4 d postinoculation. No pigs seroconverted, showed gross or microscopic lesions, or had positive IFAT results. These results provide evidence of differences in patterns of colonization between pigs sired by different boars, suggesting a possible genetic effect. 相似文献